Definition of two-spirit:
(n) :
(chiefly Canada, US) A Native North American bisexual, homosexual, or gender-variant person; especially one belonging to a traditional tribal third-gender, fourth-gender, or transgender cultural category that has a ceremonial role.
(adj) :
(chiefly Canada, US) Of, pertaining to, or being a two-spirit (noun sense).
(adj) :
(theology) Involving two spirits; especially, pertaining to the doctrine of dualism espoused in the so-called Treatise on the Two Spirits in the Dead Sea Scrolls.